Imaging Proteins Using Electrons
Imaging with electrons allows researchers to discern atomic details of large, complex molecules like proteins. During the imaging process, the interaction of electrons with the molecules being imaged inflicts damage that can alter the structure. Cryogenic temperatures (cryo-EM) can reduce the extent of this damage, but as we showed in a recent study, some parts […]
Visualizing Proteins with Cryo-EM
This image displays a number of the critical elements for determining the structure of a protein by cryo-electron microscopy. First, a solution containing many identical copies of a protein are spread on a grid (upper left), and quickly frozen to liquid nitrogen temperatures. These are imaged with an electron beam (teal), producing black-and-white images showing […]
Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, Waybright T, Juneja P, O’Neill H, Stanley CB, Bhowmik D, Ramanathan A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D. Journal of Biological Chemistry 295: 1105-19 (2020)
Cryo-electron microscopy instrumentation and techniques for life sciences and materials science
Williams R, McComb D, Subramaniam S. MRS Bulletin 44: 929-34 (2019)
Cryo-EM Structure of a Dimeric B-Raf:14-3-3 Complex Reveals Asymmetry in the Active Sites of B-Raf Kinases
Science in the City | September 26, 2019 This week we profile a recent publication in Science from the laboratory of Dr. Sriram Subramaniam at UBC.
Structure of the primed state of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome
Chittori S, Hong J, Bai Y, Subramaniam S. Nucleic Acids Research 47: 9400-09 (2019)
Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases
Kondo Y, Ognjenović J, Banerjee S, Karandur D, Merk A, Kulhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J. Science 366: 109-15 (2019)
Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9
Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonovic M, Subramaniam S. Nature Structural & Molecular Biology 26: 679-85 (2019)
Frontiers in Cryo Electron Microscopy of Complex Macromolecular Assemblies
Ognjenović J, Grisshammer R, Subramaniam S. Annual Review of Biomedical Engineering 21: 395-415 (2019)
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A. Cell Reports 27: 387-99 (2019)