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structural biology

B-raf protein as a purple and teal pair ontop of the 14-3-3 platform

Cryo-EM Structure of a Dimeric B-Raf:14-3-3 Complex Reveals Asymmetry in the Active Sites of B-Raf Kinases

Science in the City | September 26, 2019 This week we profile a recent publication in Science from the laboratory of Dr. Sriram Subramaniam at UBC.

Structure of B-Raf dimer bound to the 14-3-3 complex, where one protomer inserts its c-terminal tail into the active site of its neighbour

Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases

Kondo Y, Ognjenović J, Banerjee S, Karandur D, Merk A, Kulhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J. Science 366: 109-15 (2019)

The final cut: Cas9 editing

The final cut: Cas9 editing

Nature News & Views | July 8, 2019 The ability of CRISPR-Cas9 to accurately and efficiently target and cleave any segment of double-stranded DNA based solely on the sequence of its loaded guide RNA has revolutionized genome editing.

UBC scientists capture images of gene-editing enzymes in action

UBC scientists capture images of gene-editing enzymes in action

UBC News | July 8, 2019 Findings hold promise for future treatment of human diseases caused by DNA mutations.

Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9

Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9

Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonovic M, Subramaniam S. Nature Structural & Molecular Biology 26: 679-85 (2019)

Program in Cryo-EM Guided Drug Design
Faculty of Medicine
Djavad Mowafaghian Centre for Brain Health
Room 3404 – 2215 Wesbrook Mall
Vancouver, BC Canada V6T 1Z3
Tel 604 822 8073
Website electron.med.ubc.ca
Email cryoem.lab@ubc.ca
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