Since our first demonstration in 2006 that Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) could be a powerful tool for imaging whole cells and tissue in 3D, we have continued to refine the method.
We have applied FIB-SEM methods to a variety of important problems in cell biology that have led to unprecedented insights into subcellular architecture and cellular mechanisms.
Our current interests in the use of this technology ranges include the study of cell-cell synapses involved in viral infection, cancer progression and age-related changes in mitochondrial architecture.